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aav1 ef1a dio hchr2 h134r eyfp wpre hgh  (Addgene inc)


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    Addgene inc aav1 ef1a dio hchr2 h134r eyfp wpre hgh
    Aav1 Ef1a Dio Hchr2 H134r Eyfp Wpre Hgh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wpre+hgh/pAAV-EF1a-double+floxed-hChR2(H134R)-EYFP-WPRE-HGHpA+(Plasmid+%2320298)/bio_rxiv__64898__2026__03__27__714842-214-18-19
    Average 96 stars, based on 545 article reviews
    aav1 ef1a dio hchr2 h134r eyfp wpre hgh - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Anatomical Diversity of the Adult Corticospinal Tract Revealed by Single-Cell Transcriptional Profiling
    Article Snippet: WPRE.hGH (catalog #105553-AAVrg, Addgene) into either the cervical (C6/7) or lumbar spinal cord (L4/5), as described above.

    Article Title: Anterior Thalamic Excitation and Feedforward Inhibition of Presubicular Neurons Projecting to Medial Entorhinal Cortex
    Article Snippet: WPRE.hGH (Penn Vector Core, Addgene 26973P) with serotypes 5 or 9, contained an enhanced ChR2-EYFP fusion gene, driven by a hSynapsin promoter.

    Concentration Assay:

    Article Title: Frequency selective neuronal modulation triggers spreading depolarizations in the rat endothelin-1 model of stroke
    Article Snippet: .. WPRE.hGH (#26973, Addgene USA) was diluted in PBS to a final concentration of 3.6 109 vc/uL. ..

    Injection:

    Article Title: Increased excitatory synapse size in hippocampal place cells compared to silent cells.
    Article Snippet: .. WPRE.hGH (3.3 × 1010 GC/mL, Addgene #105553- AAV9) was injected at AP: −2, DV: −1.1, ML: 1.8 coordinates in the left hemisphere with a 2.3 nL/s flow rate in four bouts separated by 1 min pauses. ..

    Article Title: Dysregulation of the Basal Ganglia Indirect Pathway in Early Symptomatic<i>Q175</i>Huntington's Disease Mice
    Article Snippet: Arch-GFP was virally expressed in parvalbumin-expressing (PV+) GPe neurons in PV-cre mice through unilateral injection of AAV9.CBA.Flex.Arch-GFP.WPRE.SV40 (RRID: Addgene_22222; 5 1011 genome copies/ml; AP: 0.27 mm; ML: 1.90 mm; DV: 3.95 mm, 3.45 mm; 0.25ml per ventral injection and 0.20ml per dorsal injection). eGFP was virally expressed in PV1 GPe neurons in PV-cre mice through unilateral injection of AAV9.Syn.DIO.eGFP. .. WPRE.hGH (RRID:Addgene_100043; 3 1011 genome copies/ml; AP: 0.27 mm; ML: 1.90 mm; DV: 3.95 mm, 3.45 mm; 0.25ml per ventral injection and 0.20ml per dorsal injection). ..

    Plasmid Preparation:

    Article Title: Region-selective control of the thalamic reticular nucleus via cortical layer 5 pyramidal cells.
    Article Snippet: DIO.hChR2(H134R)-eYFP.WPRE.hGH (based on Addgene plasmid 20298, UNC Vector Core), AAV5.EF1.dflox.hChR2(H134R)-mCherry. .. WPRE.hGH (based on Addgene plasmid 20297, UNC Vector Core), AAV5. .. CAG.Flex.ArchT-GFP (based on Addgene plasmid 28307, UNC Vector Core) and AAV.DFO.ChR2-eYFP53 viruses were injected in the right-side neocortex or brainstem (200 nl, 1 nl s−1) using borosilicate glass capillaries.



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    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
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    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
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    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
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    aav hsyn cre  (Addgene inc)
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    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
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    aavrg hsyn cre  (Addgene inc)
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    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
    Aavrg Hsyn Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/pmc13007250-378-12-13
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    Image Search Results


    (A) Schematic of retrograde labeling of input projection neurons to the VTA using AAV.rg-hSyn1-EGFP. (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).

    Journal: bioRxiv

    Article Title: Midbrain Tet1 dosage defines inter-individual binge-eating susceptibility

    doi: 10.64898/2026.03.14.711800

    Figure Lengend Snippet: (A) Schematic of retrograde labeling of input projection neurons to the VTA using AAV.rg-hSyn1-EGFP. (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).

    Article Snippet: To perform brain-wide mapping of VTA inputs, AAV rg -hSyn1-EGFP (Addgene, #105553, 7E12 GC/mL) was bilaterally injected into the VTA of 6–8-week-old Tet1 +/– mice and littermate controls.

    Techniques: Labeling, Two Tailed Test, Expressing, One-tailed Test